Journal: Microbiology Spectrum

Article Title: C-Fiber Degeneration Enhances Alveolar Macrophage-Mediated IFN-α/β Response to Respiratory Syncytial Virus

doi: 10.1128/spectrum.02410-22

Figure Lengend Snippet: VIP regulates AM and IFN-α/β responses post-RSV infection. (a) AM percentages at 12 h postinfection in lung tissues of KCF mice with or without VIPhyb treatment. (b) IFN-α/β secretion at 12 h postinfection as measured in BALF samples from KCF mice with or without VIPhyb treatment. (c) AM percentages at 12 h postinfection in lung tissues of intact mice with or without VIP treatment. (d) IFN-α/β secretion at 12 h postinfection as measured in BALF samples from intact mice with or without VIP treatment. *, P < 0.05, and **, P < 0.01, compared with RSV or KCF-RSV group; ## , P < 0.01, and ### , P < 0.001, compared with VIP-RSV group. Data are representative of 2 independent experiments. Error bars show SEM.

Article Snippet: Both VIP and the VIP receptor antagonist VIPhyb were synthesized by GL Biochem Ltd. (Shanghai, China), with purities of >98%.

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: C-Fiber Degeneration Enhances Alveolar Macrophage-Mediated IFN-α/β Response to Respiratory Syncytial Virus

doi: 10.1128/spectrum.02410-22

Figure Lengend Snippet: VIP protects against RSV infection. (a) RSV titers in lung tissues of KCF mice with or without VIPhyb treatment. (b) Representative lung histopathological images and scores of KCF mice with or without VIPhyb treatment. Scale bar = 50 μm. (c) RSV titers in lung tissues of intact mice with or without VIP treatment. (d) Representative lung histopathological images and scores of intact mice with or without VIP treatment. *, P < 0.05, and **, P < 0.01, compared with RSV or KCF-RSV group. Data are representative of 2 independent experiments. Error bars show SEM.

Article Snippet: Both VIP and the VIP receptor antagonist VIPhyb were synthesized by GL Biochem Ltd. (Shanghai, China), with purities of >98%.

Techniques: Infection

Journal: Microbiology Spectrum

Article Title: C-Fiber Degeneration Enhances Alveolar Macrophage-Mediated IFN-α/β Response to Respiratory Syncytial Virus

doi: 10.1128/spectrum.02410-22

Figure Lengend Snippet: Sequences of VIPhyb and VIP

Article Snippet: Both VIP and the VIP receptor antagonist VIPhyb were synthesized by GL Biochem Ltd. (Shanghai, China), with purities of >98%.

Techniques: Sequencing

Journal: Neurobiology of disease

Article Title: P2RX7 modulates the function of human microglia-like cells and mediates the association of IL18 with Alzheimer’s disease traits

doi: 10.1016/j.nbd.2025.107106

Figure Lengend Snippet: Extracellular ATP induces IL1β and IL18 gene expression upregulation in a P2RX7-dependent manner. A) IL1β , IL18 , and NLRP3 expression levels were measured with qPCR from MDMi treated with DMSO alone (Ctrl), 1 mM ATP (ATP), or 1 mM ATP plus 10 μM A740003 (ATP + A74). Statistics were determined with a repeated measure one-way paired ANOVA with Newman-Keuls multiple comparisons test. N = 14. B) IL-1β ( N = 6) and IL-18 ( N = 5) protein detection in supernatants of MDMi treated with DMSO alone (Ctrl), 1 mM ATP (ATP), or 1 mM ATP plus 10 μM A740003 (ATP + A74). Statistics were determined with a two-way paired ANOVA. C) IL1β , IL18 , and NLRP3 expression levels were measured with qPCR from MDMi treated with DMSO alone (Ctrl), or 500 μM bzATP (bzATP). Statistics were determined with a paired t -test. N = 9. ns = p > 0.05, * p ≤ 0.05, ** ≤ 0.01,*** ≤ 0.001, **** ≤ 0.0001.

Article Snippet: The A740003 P2RX7 antagonist (Tocris biotechne, #861393–28–4, Batch #3B/266155) was dissolved in DMSO and added to MDMi cultures at a concentration of 10 μM.

Techniques: Gene Expression, Expressing

Journal: Neurobiology of disease

Article Title: P2RX7 modulates the function of human microglia-like cells and mediates the association of IL18 with Alzheimer’s disease traits

doi: 10.1016/j.nbd.2025.107106

Figure Lengend Snippet: ATP-mediated P2RX7 activation reduces MDMi Aβ1–42 uptake abilities. A) Mean fluorescence intensity (MFI) of HyLite Fluor 647-labeled Aβ1–42 peptide uptake normalized to DAPI MFI in MDMi treated with DMSO alone (Ctrl), 1 mM ATP (ATP), or 1 mM ATP and 10 μM A740003 (ATP + A74), measured by fluorescence on a microplate reader. Data are represented relative to the control condition. Statistics were determined with a repeated measure one-way paired ANOVA with Newman-Keuls multiple comparisons test. N = 26. B) MFI of HyLite Fluor 647-labeled Aβ1–42 peptide uptake normalized to DAPI MFI in MDMi treated with DMSO alone (Ctrl), or 500 μM bzATP (bzATP), as measured by fluorescence on a microplate reader. Data are represented relative to the control condition. Statistics were determined with a paired t-test. N = 14. C) Mean fluorescence intensity (MFI) of HyLite Fluor 647-labeled Aβ1–42 per DAPI+ cell in MDMi imaged using confocal microscopy. MDMi were treated with DMSO alone (Ctrl), 1 mM ATP (ATP), or 1 mM ATP and 10 μM A740003 (ATP + A74). Statistics were determined with a repeated measure one-way paired ANOVA with Newman-Keuls multiple comparisons test. N = 12. D) Representative images of MDMi treated with DMSO alone (Ctrl), 1 mM ATP (ATP), or 1 mM ATP and 10 μM A740003 (ATP + A74) and stained for P2RX7 (green) and DAPI (blue) and used to measure uptake of HyLite Fluor 647-labeled Aβ1–42 peptide (red). Images were processed in CellProfiler. ns = p > 0.05, *p ≤ 0.05, ** ≤ 0.01,*** ≤ 0.001, **** ≤ 0.0001.

Article Snippet: The A740003 P2RX7 antagonist (Tocris biotechne, #861393–28–4, Batch #3B/266155) was dissolved in DMSO and added to MDMi cultures at a concentration of 10 μM.

Techniques: Activation Assay, Fluorescence, Labeling, Control, Confocal Microscopy, Staining

Journal: Neurobiology of disease

Article Title: P2RX7 modulates the function of human microglia-like cells and mediates the association of IL18 with Alzheimer’s disease traits

doi: 10.1016/j.nbd.2025.107106

Figure Lengend Snippet: IL18 but not IL1β is correlated with P2RX7 gene expression in MDMi. IL1β , IL18 , and P2RX7 expression levels were measured with qPCR from MDMi. A) Correlation of IL1β and P2RX7 expression. Pearson correlation coefficient (r) = −0.002, two-tailed P value = 0.99. B) Correlation of IL18 and P2RX7 expression. Pearson correlation coefficient (r) = 0.59, two-tailed P value = 0.02. R.E. = Relative Gene Expression (2^−(ΔCt)*1000) to GAPDH . N = 15.

Article Snippet: The A740003 P2RX7 antagonist (Tocris biotechne, #861393–28–4, Batch #3B/266155) was dissolved in DMSO and added to MDMi cultures at a concentration of 10 μM.

Techniques: Gene Expression, Expressing, Two Tailed Test